BARD1 levels were downregulated in cavin3 KO cells (Amount 1figure product 1D). (206K) GUID:?841BB1E2-1F3C-4F78-A578-0D9D845FF098 Figure 1figure product 1source data 3: Raw western data for HeLa WT, cavin3 KO, and cavin3 KO with PRKM1 cavin3-GFP cells with molecular weight markers for Figure 1figure product 1C. (A) Western blot analysis of anti-rabbit cavin3, (B) anti-rabbit CAV1, (C) anti-mouse GFP, (D) anti-rabbit DDX21, (E) anti-rabbit Caldesmon, and (F) anti-Tubulin antibodies in (1) HeLa WT, (2) cavin3 KO cells, and (3) cavin3KO?+?cavin3 GFP-expressing cells. elife-61407-fig1-figsupp1-data3.pdf (285K) GUID:?72BB3FE7-3E63-42B6-A726-A95CB14600DD Number 4source data 1: Natural western data for MCF7 cells with molecular weight markers for Number 4C. (A) Western blot analysis of anti-rabbit BRCA1, (B) anti-mouse Tubulin, and (C) anti-mouse GFP antibodies in (1) GFP lysates, (2) cavin1-GFP lysates, and (3) cavin3-GFP lysates. elife-61407-fig4-data1.pdf (255K) GUID:?0A24A2C3-0754-46D1-81A1-F6E8C595A45D Number 4source data 2: Natural western data for MCF7 cells with molecular excess weight markers for Number 4D. (A) Western blot analysis of anti-mouse GFP, (B) anti-rabbit BRCA1, and (C) anti-mouse Tubulin antibodies in (1) MCF7/GFP untreated, (2) MCF7/GFP?+?MG132-treated lysates, and (3) MCF7/cavin3-GFP untreated lysates. elife-61407-fig4-data2.pdf (232K) GUID:?A4A72304-368A-404A-932B-E55205B9A08B Number 4source data 3: Natural western data for A431 cells with molecular excess weight markers for Number 4E. (A) Western blot analysis of anti-rabbit cavin3, (B) anti-rabbit CAV1, (C) anti-mouse Tubulin, and (D) anti-rabbit BRCA1 antibodies in (1) A431 cells treated with control siRNA oligos, (2) A431 cells treated with cavin3-specific siRNA oligo 1, and (3) A431 cells treated with cavin3-specific siRNA oligo 2. elife-61407-fig4-data3.pdf (583K) GUID:?2D86ACCD-17BC-4EE2-BC81-721E13A250CE Number 4source data 4: Natural western data for A431 cells with molecular weight markers for Number 4F. (A) Western blot analysis of anti-rabbit BRCA1, (B) anti-mouse Tubulin, (C) anti-rabbit cavin3, and (D) anti-rabbit CAV1 antibodies in A431 cells treated with (1) control siRNA oligos, (2) A431 cells treated with BRCA1-specific siRNA oligo 1, and (3) A431 cells treated with BRCA1-specific siRNA oligo 2. elife-61407-fig4-data4.pdf (346K) GUID:?2D416514-6EFE-48BE-9166-9A05DA861468 Figure 4source data 5: Raw western data for A431 cells with molecular weight markers for Figure 4G. (A) Western blot analysis of anti-rabbit BRCA1, (B) anti-rabbit cavin3, and (C) anti-mouse Tubulin antibodies in (1)?A431 cells treated with control siRNA (control KD) oligos no treatment, (2) A431 cells treated with control siRNA oligos (control KD) and MG132 for 6 hr, (3) cavin3-specific siRNA (cavin3 KD) oligo 1 no treatment, (4) cavin3-specific siRNA (cavin3 KD) oligo 1 and MG132 for 6 hr, (5) cavin3-specific siRNA (cavin3 KD) oligo 2 no treatment, and (6) cavin3-specific siRNA (cavin3 KD) oligo 2 and MG132 for 6 hr. elife-61407-fig4-data5.pdf (881K) GUID:?D6095585-2176-468B-961A-BFA2408122DA Number 4figure supplement 1source data 1: Natural western data for HeLa WT and cavin3 KO cells with molecular weight markers for Number 4figure supplement 1E. (A) Western blot analysis of anti-rabbit LJI308 cavin3, (B) anti-mouse Tubulin, (C) anti-rabbit CAV1, and (D) anti-rabbit BRCA1 antibodies in (1) MCF7, (2) MDA-MB231, (3) A431, and (4) HeLa cells. elife-61407-fig4-figsupp1-data1.pdf (529K) GUID:?5F331204-7925-4E69-9ED4-BA8FE6EDEE58 Figure 4figure product 2source data 1: Raw western data for MDA-MB231 cells with molecular weight markers for Figure 4figure product 2A. (A) Western blot analysis of anti-rabbit BRCA1, (B) anti-rabbit cavin3, and (C) anti-mouse Tubulin in (1) MDA-MB231 treated with control siRNAs, (2) MDA-MB231 cells treated with cavin3-specific siRNA oligo 1, and (3) MDA-MB231 cells treated with cavin3-specific siRNA oligo 2. elife-61407-fig4-figsupp2-data1.pdf (601K) GUID:?490DC542-2429-48D0-AC98-38A6A16A21DF Number 4figure product 2source data 2: LJI308 Natural western data for MDA-MB231 cells with molecular excess LJI308 weight markers for Number 4figure product 2B. (A) Western LJI308 blot analysis of anti-rabbit CAV1, (B) anti-rabbit cavin3, (C) anti-rabbit cavin1, (D) anti-mouse Tubulin, (E) anti-rabbit BRCA1, and (F) anti-mouse BRCA1 in (1) MDA-MB231 cells treated with LJI308 control siRNAs, (2) MDA-MB231 cells treated with BRCA1-specific siRNA oligo 1,.
