The decreased neutralization effect is of concern and requires further surveillance and epidemiological studies. 5. sera obtained from people vaccinated with Sputnik V in relation to internationally relevant genetic lineages B.1.1.7, Tuberstemonine B.1.351, P.1, B.1.617.2, B.1.617.3 and Moscow endemic variants B.1.1.141 (T385I) and Rabbit Polyclonal to ADA2L B.1.1.317 (S477N, A522S) with mutations in the RBD domain has been assessed. The data obtained indicate no significant differences in VNA against B.1.1.7, B.1.617.3 and local genetic lineages B.1.1.141 (T385I), B.1.1.317 (S477N, A522S) with RBD mutations. For the B.1.351, P.1, and B.1.617.2 statistically significant 3.1-, 2.8-, and 2.5-fold, respectively, VNA reduction was observed. Notably, this decrease is lower than that Tuberstemonine reported in publications for other vaccines. However, a direct comparative study is necessary for a conclusion. Thus, sera from Sputnik V-vaccinated retain neutralizing activity against VOC B.1.1.7, B.1.351, P.1, B.1.617.2, B.1.617.3 as well as local genetic lineages B.1.1.141 and B.1.1.317 circulating in Moscow. strong class=”kwd-title” Keywords: COVID-19, SARS-CoV-2, vaccine, Sputnik V, VOC, virus neutralizing activity 1. Tuberstemonine Introduction The recent successful launch of SARS-CoV-2 vaccines gives hope for Tuberstemonine an early reduction of the pandemic and a return to the pre-quarantine living conditions [1,2,3,4,5]. All major vaccines ensure a convincing level of protection (over 90%) in the short term and reliable protection against the severe course of COVID-19 according to the clinical trials results. For countries leading the universal immunization program, the downward trend in disease incidence and mortality is evident [6]. The statistics data for Israel, the UAE, the USA, and the UK show a sharp decline in morbidity and mortality from COVID-19 after reaching 30 doses per 100 people [7]. It is not clear how a similar level of protection for widely used vaccines can be maintained for new SARS-CoV-2 strains with mutations in the SARS-CoV-2 Spike (S; envelope glycoprotein). Since most vaccines include Spike as a principal immunogen, its variability surveillance can timely inform on the risks of escape from neutralizing antibodies formed by vaccination. Currently, over a million of SARS-CoV-2 genomic Tuberstemonine sequences are available on the GISAID server [8]. Individual virus variants with more pronounced epidemiological, immunological, or pathogenic properties, are of concern. At the beginning of 2021, the list of variants of concern (VOC) included the B.1.1.7, B.1.351, P.1; currently, this list already contains ten records including linages B.1.617.1, B.1.617.2, B.1.617.3 [9]. For some VOC, identical mutations in S proteins appear independently, requiring in-depth research into their effects on transmissibility, severity, and ability to overcome immunity formed in convalescents and vaccinated. Active study of the composition of the mutations that have been accumulating recently in S protein shows that most of them increase interaction with the hosts ACE2 receptor [10]. The neutralizing effect of serum samples obtained from patients vaccinated with Pfizer/BNT162b2 was reduced for the B.1.351, B.1.1.28, and B.1.617 variants by 7.85, 5.12, and 3 times, respectively, in comparison to the wild-type virus [11,12]. Assessment of individual mutation contributions shows the major impact of the E484K mutation [11,13]. A comparative study of the decrease in neutralizing activity for serum samples obtained from the patients vaccinated with Moderna/mRNA-1273 and those vaccinated with Pfizer/BNT162b2 showed 20- and 40-fold decrease respectively against B.1.351 [14]. In fact, the cross-neutralization of the B.1.351 variant was comparable to SARS-CoV-1 and Bat SL-CoV-WIV1, suggesting that a relatively small number of Spike mutations may result in the escape from neutralizing antibodies. It is becoming apparent that mutations in RBD can pose the greatest risk, both of making SARS-CoV-2 more contagious and of reducing antibody neutralization. These mutations are likely to include K417N, L452R, E484K, S494P and N501Y/T, based on molecular dynamics data [15]. We have previously developed and tested the Sputnik V vaccine, which forms the high titers of neutralizing antibodies and.
