The lysates were subjected to immunoprecipitation with GFP-Trap beads

The lysates were subjected to immunoprecipitation with GFP-Trap beads. a novel bad feedback on mTORC1. Our findings uncover ZNRF2 as a component of the amino acid sensing machinery that functions upstream of Rag-GTPases and the V-ATPase to activate mTORC1. DOI: http://dx.doi.org/10.7554/eLife.12278.001 PAT1 (proton-assisted transporter 1) (Bar-Peled and Sabatini, 2014; Bar-Peled et al., 2012; Nada et …
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